32d mouse lymphoblast cells  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Potassium Iodide Induces Apoptosis in Salivary Gland Cancer Cells

doi: 10.3390/ijms26115199

Figure Lengend Snippet: KI reduces the viability of SLC5A5-expressing SGC cells. ( A , B ) The fold change in SLC5A5 expression was determined by qPCR in ( A ) human submandibular SG epidermoid carcinoma (A253; n = 5), myeloid hematopoietic (HL-6; n = 2, U937; n = 2), human, and murine endothelial, respectively (HUVEC; n = 2, BMEC1; n = 3), as well as in (HT1080; n = 3) fibrosarcoma cells and ( B ) murine lymphoblast (32D; n = 2) cells, fibroblasts (MS-5; n = 2, 3T3; n = 2), and submandibular SG adenocarcinoma (WR21; n = 3) cells. SLC5A5 gene expression levels were normalized to BETA-ACTIN expression in the same samples, and fold changes were adjusted relative to the expression levels in control samples. ( C , D ) The iodine concentration was assessed in cell lysates of A253 ( C ) and WR21 ( D ) cells 48 h after KI treatment at the indicated concentrations ( n = 4/5 per group). ( E ) Representative light microscopy images of murine WR21 and human A253 SGC cells 48 h after incubation with or without KI (100 μM; scale bar = 100 μm). ( F ) The viability rate of A253 cells treated with the indicated KI concentrations for 48 h was determined by trypan blue exclusion ( n = 8 for the control (co) group and n = 3 for KI 25, 50, 100, and 200 μM groups). ( G , H ) The absolute number of viable and control (co) A253 ( G ) and WR21 cells ( H ) after 48 h in culture, following the addition of KI (100 μM), was determined using the trypan blue exclusion assay ( n = 10 and 5/group for A253 cells and n = 4, 3/group for WR21 cells). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 using a one-way ANOVA test (to determine the effects of two independent variables on a dependent variable) or Student’s t -test (to compare the performance of two groups under different conditions), with mean ± SD depicted.

Article Snippet: The basic media for the human submandibular SG epidermoid carcinoma (A253) cells (Cat. HTB-41, ATCC, Manassas, VA, USA) was McCoy’s 5A (Modified) Medium (Cat. 16600082, Gibco, Grand Island, NY, USA); for U-937 human histiocytic lymphoma cells (Cat. CRL-1593.2, ATCC, Manassas, VA, USA), it was RPMI-1640 medium (Cat. 11875093, Gibco, Grand Island, NY, USA); for HL-60 human promyeloblast cells (Cat. CCL-240, ATCC, Manassas, VA, USA), it was IMDM (Cat. 12440053, Gibco, Grand Island, NY, USA); for HUVEC human umbilical venule endothelial cells (Cat. CRL-1730, ATCC, Manassas, VA, USA), it was F-12K Medium (Cat. 21127022, Gibco, Grand Island, NY, USA) supplemented with heparin (Cat. H3393, Sigma, Saint Louis, MO, USA) and ECGS (Cat. CB-40006, Fisher Scientific, Waltham, MA, USA); for BMEC human bone marrow microvascular endothelial cell (Cat. CRL-3421, ATCC, Manassas, VA, USA), it was MCDB-131 medium (Cat. 10372-019, Gibco, Grand Island, NY, USA); for HT-1080 human fibrosarcoma cells (Cat. CCL-121, ATCC, Manassas, VA, USA), it was MEM (Cat. 137-17215, Wako, Osaka, Japan); for WR21 mouse submandibular SG adenocarcinoma cells (Cat. CRL-2189, ATCC, Manassas, VA, USA) and for murine NIH/3T3 fibroblast (Cat. CRL-1658, ATCC, Manassas, VA, USA), it was D-MEM medium (Cat. 044-29765, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan); for 32D mouse lymphoblast cells (Cat. CRL-11346, ATCC, Manassas, VA, USA), it was RPMI 1640 (Cat. 11875093, Gibco, Grand Island, NY, USA); and for MS5 murine stromal cells (kindly provided by Dr. MAS Moore, Sloan Kettering Cancer Center, New York, NY, USA), it was IMDM (Cat. 12440053, Gibco, Grand Island, NY, USA).

Techniques: Expressing, Gene Expression, Control, Concentration Assay, Light Microscopy, Incubation, Trypan Blue Exclusion Assay